重組蛋白雜合通用 I 型干擾素由 PBL Assay Science(目錄號(hào) #11200-1)生產(chǎn),僅供研究使用 (RUO),包裝為 1e5 (100,000) 單位/瓶。這種細(xì)胞因子在多個(gè)物種中表現(xiàn)出活性,對(duì)于與物種相關(guān)的 I 型 IFN-α 不可用或表現(xiàn)不一致的體內(nèi)或體外研究至關(guān)重要。
商品詳情參考文獻(xiàn)相關(guān)資料
產(chǎn)品介紹
品牌
PBL Assay Science
貨號(hào)
11200-1
品名
Human IFN Alpha Hybrid Protein (Universal Type I IFN)
規(guī)格
1 x 105 units
分子量
19.4 kDa
來源
Construct described in Rehberg, E., (1982) J. Biol. Chem. 257: 11497 derived from E.coli
純度
> 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
生物活性
Measured using a cytopathic inhibition assay on Bovine Kidney Cells (MDBK) with Vesicular Stomatis Virus (VSV); human lung carcinoma cell line A549 with encephalomyocarditis virus (EMCV); Mouse (L929) cells with EMCV
緩沖液
Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
內(nèi)毒素
< 1 EU/μg
純度
> 95%
純化方法
Purified from E. coli by a combination of hydrophobic interaction, ion exchange and gel
permeation chromatography
貯存
For retention of full activity store at -70 oC or below and avoid repeated freeze-thaw cycles