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Mouse Interleukin 35 (IL35) CLIA Kit
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貨號(hào):EKU09968
規(guī)格:96T
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Mouse Interleukin 35 (IL35) CLIA Kit

商品詳情 參考文獻(xiàn) 相關(guān)資料

Product Description

Species Reactivity Mus musculus (Mouse)
Format 96 well plate
Detection Range 15.62-1000 pg/mL
Sensitivity 0.52 pg/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Time 2 hours, 40min
UniProt P43431
Gene ID 16159
Gene Name Interleukin 35
Application CLIA
Assay Type Sandwich
Detection Method Chemiluminescent
Shipping Condition Blue ice
Storage Instructions -20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date 8 months
Specificity This assay has high sensitivity and excellent specificity for detection of Interleukin 35 (IL35). No significant cross-reactivity or interference between Interleukin 35 (IL35) and analogues was observed.
Test Principle The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 35 (IL35). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 35 (IL35). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 35 (IL35) level in the sample or standard.;
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 35 (IL35) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high leve
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions For research use only. Not for diagnostic procedures.
Quality Systems The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

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