The Badrilla CAPTUREome^TM S-Palmitoylated Protein Kit is a simple and easy way of determining whether your protein of interest is post translationally modified with a palmitate group. The assay is based on the acyl-RAC (resin assisted capture) method and facilitates, with four simple steps – Block, Cleave, Capture and Analysis - determination of protein palmitoylation.

Kit highlights

• Enables capture of S-palmitoylated proteins.
• Kit designed to process 4 x 5 biological samples with paired negative controls (i.e. 40 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.

References

1. Forrester M.T., et al. (2011) Site-specific analysis of protein S-acylation by resin assisted capture. J. Lipid Res. 52: 393-398
2. Howie, J. et al (2014) Substrate recognition by the cell surface palmitoyl transferase DHHC5. PNAS 111: 17534–17539

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