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APC - anti mouse B220/CD45R - Clone RA3-3A1
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APC - anti mouse B220/CD45R - Clone RA3-3A1
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APC - anti Mouse B220/CD45R - RA3-3A1

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73,00 € tax excl.

142,00 € tax excl.

Product Description

  • RA3-3A1
  • Rat IgG2a
  • Flow Cytometry
  • APC
  • 0.2 mg/ml
  • Mouse cell line RAW 112
  • Mouse
  • The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • RUO
  • Liquid
  • Store in the dark at 2-8 °C
Mouse APC Vial View larger
CD45R antibody (clone RA3-6B2) has been reported to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. It is expressed on B lymph ocytes at all stages from pro-B through mature and activated B cell, but it is decreased on plasma cells and a subset of memory B cells. The levels of CD45R expression on the B-cell lineage appear to be developmentally regulated. It is also reportedly found on the abnormal T cells involved in the lymph adenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymph okine-activated killer cells (NK cells and non-MHC-restricted CTL), on apoptotic T lymph ocytes of mice injected with bacterial superantigen, on a population of NK-cell precursors in the bone marrow, and on B-lymph ocyte, T-lymph ocyte, and macrophage progenitors in fetal liver. The CD45R antigen has been reported not to be on hematopoietic stem cells, naive T lymph ocytes, or MHC-restricted CTL. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. CD45R is commonly used as a pan B-cell marker; however, CD19 expression, detectable by CD19 antibody (clone 1D3), is reported to be more restricted to the B-cell lineage. CD45R antibody (clone RA3-6B2) has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses. Cross-reaction of the RA3-6B2 clone with activated human T lymph ocytes has also been reportedly observed.
  • B220
  • 180-240 kD
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