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包含：PE-labeled immunoglobulin in 20 mM Tris buffer with 137 mM NaCl, 0.5% BSA and 0.09% (w/v) sodium azide

特異性：

The Wug.E9 antibody reacts with mouse P-selectin (CD62P, PADGEM, GMP-140), a 140-kDa single-chain polypeptide belonging to the selectin family of adhesion molecules1 . P-selectin is expressed in the a-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cellular activation, P-selectin is translocated to the surface membrane. P-selectin mediates the adhesion of activated platelets to neutrophils and monocytes2 . The surface expression of P-selectin on platelets can be used as a marker for a-granule secretion.

用途：

The antibody preparation is optimized for flow cytometric applications: Use 5 μl to stain ~106 platelets or ~0.5x106 cells in a recommended volume of 25 μl. Incubate for 15 minutes at room temperature, stop reaction by addition of 400 μl PBS and analyze samples within 30 minutes. For immunofluorescent staining of acetone-fixed frozen sections, the appropriate dilution must be determined individually. 

制備和儲(chǔ)存：

The antibody was purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography. The antibody was conjugated with PE under optimum conditions. The solution is free of unbound PE. Store product undiluted at 4°C and avoid prolonged exposure to light. Stable for one year from date of shipment. Do not freeze.

注意：

 Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer.

注：僅供研究使用，不用于診斷或治療用途。