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Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM155; H3K36me3, Histone H3 (trimethyl K36); ChIP Validated

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Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM155; H3K36me3, Histone H3 (trimethyl K36); ChIP Validated

品牌：RevMAb

貨號(hào)：31-1051-00

規(guī)格：100 ug

貨期：電詢

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Product Details
Product Name	Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody [RM155]
Product Description	Rabbit monoclonal to Trimethylated Histone H3 Lysine 36, H3K36me3, Histone H3 (trimethyl K36)
Catalog No.	31-1051-00
Clone Name	RM155
Product Data Sheet	Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody Data Sheet RM155
Specificity	RM155 reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with monomethylated Lysine 36 (K36me1), dimethylated Lysine 36 (K36me2), or other methylations in histone H3.
Immunogen	A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys36).
Application	Western Blot (WB) Chromatin IP (ChIP) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.5 ug/mL – 2 ug/mL; ChIP: 2 ug/mL- 10 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
	RM155 specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non- modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.
	Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM155 at 2.0 ug/mL, showed a band of histone H3 trimethylated at Lysine 36 (K36me3) in HeLa cells.
	ChIP performed on HeLa cells using H3K36me3 antibody (RM155, 5ug). Real-time PCR was performed using primers specific to the gene indicated.