Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20ng/lane), using 0.2ug/mL of RevMAb clone RM106. This antibody reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).
ELISA of mouse immunoglobulins shows RM106 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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