RM188 reacts to both Histone H3.1 and Histone H3.3.
Western Blot of recombinant Histone H3.3 and Histone H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using RM188 at 0.025 ug/mL, showed a band of recombinant H3.1 and H3.3 proteins, and total histone H3 in A375, HEK293, HeLa and SK-MEL-2 cells.
Immunocytochemistry of HeLa cells, using Histone H3 Rabbit mAb RM188 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K9ac (RM161, 1 ug/mL) as the detection antibody.
Sandwich ELISA against acetylated histone H3 at Lys 79 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3K79ac (RM156, 5 ug/mL) as the capture antibody and biotinylated anti-H3CT (RM188, 1 ug/mL) as the detection antibody.
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