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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone RM108
品牌:RevMAb
貨號:31-1004-00
規(guī)格:100ug
貨期:電詢

商品詳情 參考文獻 相關(guān)資料

Product Details
Product Name Anti-Mouse IgG2b Rabbit Monoclonal Antibody [RM108]
Product Description Rabbit monoclonal to Mouse IgG2b
Catalog No. 31-1004-00
Clone Name RM108
Product Data Sheet Anti-Mouse IgG2b Rabbit Monoclonal Antibody Data Sheet RM108
Specificity RM108 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
The Fc region of RM108 has been engineered to eliminate Fc receptor binding.
Immunogen Mouse IgG2b
Application Western Blot (WB) (stronger on nonreduced)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Mouse
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
.
Product Images
Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG2b (20ng/lane), using 0.2ug/mL of
RevMAb clone RM108. This antibody reacts to
nonreduced IgG2b (~150 kDa) much stronger
than the reduced γ2b form (~50 kDa).

ELISA

ELISA of mouse immunoglobulins shows RM108
reacts to the Fc region of mouse IgG2b; no cross
reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE,
human IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was
used as the primary antibody. An alkaline phosph-
atase conjugated anti-rabbit IgG as the secondary
antibody.

A

A titer ELISA of mouse IgG2b. The plate was
coated with different amounts of mouse IgG2b.
A serial dilution of RM108 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.


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