產(chǎn)品介紹
KILR® Retroparticles for Suspension Cells (Hygromycin B)
97-0006
4 Vials x 1.0 mL
KILR® Retroparticles for Suspension Cells (Hygromycin B) contains MMLV particles, that can transduce a wide variety of suspension cells with a housekeeping gene tagged with ProLabel® (ePL), a β-gal reporter fragment. This results in a high level of expression of the fusion protein inside the target cells. Target cell death in a cytotoxicity assay results in the release of the ePL-tagged protein into the medium. Addition of KILR detection reagent, containing the complementing β-gal reporter fragment, Enzyme Acceptor (EA), results in complementation of the two enzyme fragments (EA and ePL). The resulting functional enzyme hydrolyzes a substrate to generate a chemiluminescent signal.
KILR® Retroparticles (HYGRO) Suspension Part #30-566
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
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