產(chǎn)品介紹
KILR® Retroparticles for Suspension & Adherent Cells (Hygromycin B)
97-0008
2 Vials x 0.5 mL (adherent cells), 2 Vials x 0.5 mL (suspension cells)
KILR® Retroparticles for Adherent and Suspension Cells (Hygromycin B) contains two types of MMLV particles, that can transduce adherent cells and suspension cells respectively. This leads to a high level expression with a housekeeping gene tagged with β-gal reporter fragment, ProLabel® (ePL) inside the target cells. Target cell death in a cytotoxicity assay results in the release of the ePL-tagged protein into the medium. Addition of KILR detection reagent, containing the complementing β-gal reporter fragment, EA, results in complementation of the two enzyme fragments (EA and ePL). The resulting functional enzyme hydrolyzes a substrate to generate a chemiluminescent signal.
KILR Retroparticles (Hygro) Adherent & KILR Retroparticles (Hygro) Suspension
The KILR Retroparticles provide a potent vehicle for the stable delivery of the KILR reporter construct into almost any dividing cell line, enabling the creation of KILR cytotoxicity assays in the cell line expressing the target of your choice. The KILR Cytotoxicity cell line you generate enables the development of a simple, non-radioactive and dye-free method to specifically measure target cell death in a co-culture. The KILR assay has several applications in iOnc Cancer Immunotherapy (iOnc) drug development including measuring target cell death through Antibody Dependent Cellmediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), Antibody Dependent Cell Phagocytosis (ADCP), cytotoxic T cell lymphocyte mediated death (CTL), Bi-specific Antibody Mediated T Cell Redirection, Chimeric Antigen Receptor T cell (CAR-T) and adoptive T cell therapies. This assay can drive all aspects of the drug discovery process, from screening to characterization, and the development of a QC lot release assay for the immunotherapy drugs.
產(chǎn)品貯存來電咨詢更詳細(xì) 400-968-7988
(<24 h): Store at -80°C
(>24 h): Store in vapor phase of liquid nitrogen.
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