產(chǎn)品介紹
Affinity purified mouse monoclonal anti Na+-Ca2+ exchanger protein (cardiac)
3RF1pur / R3F1PUR
100 μl
with 100 μl
The antibody 3RF1pur was purified with Protein-G from in vitro produced monoclonal antibody 3RF1 directed against Na+-Ca+ exchanger protein (see product description 3RF1 for further details concerning immunoblot and immunohistochemistry). The antibody concentration is of 0.18 mg/ml in 0.1M phosphate buffered saline, pH 7.3.
Immunohistochemistry: we suggest using a dilution of 1:10’000 (0.018 ug/ml) for immunostaining with the avidin-biotin method on cryostat or paraffin-sections of paraformaldehyde (4%) or formalin-fixed tissue.
We recommend that the optimal dilutions be determined by titration experiments.
For long storage, keep small aliquots of 1-2 μl at - 80°C (or at least - 20°C). For continuous use keep diluted solutions at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
Porzig H., Li Z., Nicoll D.A., Philipson K.D. (1993) Mapping the cardiac sodium-calcium exchanger with monoclonal antibodies. Am. J. Physiol. 265:C78 -C756, 1993.
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